Read-out would be in minutes - timer in App - read by camera in your smart phone. No other machines. Links to telemedicine groups for positive results. Built in positive control - e.g. no color = improper test, green NEGATIVE, red POSITIVE.
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I’ve done my best creating when faced by a challenge. Next gen sequencing when my son went to new born intensive care - #454
@iontorrent. Ultrasound on a chip@ButterflyNetInc when wanted to monitor my daughter’s kidneys.Show this thread -
It’s clear you could do a test with a nosal swab, and then dip in a series of 3 tubes: 1) Lyse the virus and release its unique generic code - in this case an RNA molecule. 2) recognize the COVID-19 unique code with a DNA primer specific for this dangerous strain.
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Still in tube 2, reverse transcribe the COVID-19 generic material into DNA. DNA is super stable and easy to amplify so we can detect it. Move swab to 3) and use an isothermal amplification method that doesn’t need a machine and produces a color change. Use camera to read color.
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Ok. Now that we can do it with a swab and 3 tubes and a smart phone. Review each step and see how we can have the reagents already on the swab, and activated my moisture so we don’t need all the tubes, complexity and chance for failure.
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Would go for approval as a “risk assessment” NOT
#diagnostic for faster regulatory clearance. And work with regulatory agencies to validate the PROCESS so for the next TEST and NEXT#pandemic we could update without going back to regulators as long as we followed same process.Show this thread -
I’ve made some general progress on the sample collection, and
#Coronavirus detection side, and have a potential manufacturer that wants to make. Would need to work out test details today. And start bench experiments.Show this thread -
Trying to figure out how to do
#COVID19 test in one tube, so people at home don’t “mess” it up. But I also just figured out how to do an internal control and get two colors, so it’s clear when test is done right.Show this thread -
Ok, so should I work to quickly develop and deploy a simple & rapid home use test for
#Coronavirus#COVID19? Realize all the issues with regulatory approvals I’ll face in many countries. And that any test will have BOTH false negative and FALSE POSITIVES.@gatesfoundationShow this thread -
Yes. I will need help. Would be great to have an
#iOS and#android app that used the camera to read the color. And collected data in#HIPAA (medical record legal complaint way) and with#geotags produced real time map. I still need to work out how to do#COVID19 TEST in one tube!pic.twitter.com/FZyHm1AkKNShow this thread -
Background: The gold standard test for
#coronavirus is a RTQPCR reaction (that just means exponential amplification of#COVID19 specific target on this case) to see if the virus was “there”. ~$25,000 machine + dozen steps by technician and ~two hours to run tests and get results.pic.twitter.com/ZmTEQ8M609Show this thread -
Ran our At-Home Covid test today. Swabbed my nostril. Dipped in collection tube. Placed in base-station. Read out results. Collection control worked - confirms test was run correctly. Nice peace of mind. Not positive for
#Covid19. Best peace of mind.pic.twitter.com/mACjuUPmgiShow this thread -
115 days later. Production articles of our at-home, accurate, rapid, Nucleic acid Test for
#COVID19. Working on data for regulatory approvals. But not waiting to scale, we are in production now to supply millions per month starting this summer. *NOT for sale in USA YET! pic.twitter.com/R1q7WONqxNShow this thread -
It’s a platform. So
#COVID19 now. Add Flu before the fall. And additional viral or other pathogens as needed. **** working on FDA and other regulatory approvals so NOT for SALE in The U.S.A. YET ****pic.twitter.com/B9GMIiMbYOShow this thread
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